NAD+ · Dosing & maths
NAD+ dosage: what the research used
This page collects the doses reported in published NAD+ research and works through the reconstitution maths for common vial sizes. It does not recommend any dose.
Doses used in published research
Human trials of precursors used oral NR at 250–2,000 mg/day and NMN at 250–900 mg/day. IV NAD+ studies infused hundreds of milligrams slowly over several hours. Injectable NAD+ dosing for humans has not been established in controlled trials.
NAD+ reconstitution table
Syringe units (U-100: 100 units = 1 mL) needed for a given amount, by vial size and water volume:
| Vial | Water | Concentration | 25 mg | 50 mg | 100 mg |
|---|---|---|---|---|---|
| 100 mg | 2 mL | 50 mg/mL | 50 units | 100 units | > 1 mL |
| 100 mg | 5 mL | 20 mg/mL | > 1 mL | > 1 mL | > 1 mL |
| 100 mg | 10 mL | 10 mg/mL | > 1 mL | > 1 mL | > 1 mL |
| 500 mg | 2 mL | 250 mg/mL | 10 units | 20 units | 40 units |
| 500 mg | 5 mL | 100 mg/mL | 25 units | 50 units | 100 units |
| 500 mg | 10 mL | 50 mg/mL | 50 units | 100 units | > 1 mL |
NAD+ reconstitution calculator
Vial presets:
Arithmetic aid for laboratory reconstitution. It does not recommend any amount for any use.
Handling and storage
Reconstitute with bacteriostatic water, adding it slowly down the vial wall and swirling rather than shaking — see the reconstitution guide. Once mixed, refrigerate at 2–8 °C; unmixed lyophilised powder keeps longest frozen (storage guide). Converting between units, mL and mg is covered in syringe units explained.
NAD+ dosing FAQs
How much bacteriostatic water for a 100 mg NAD+ vial?
There is no fixed amount — the volume sets the concentration. Adding 5 mL to a 100 mg vial gives 20 mg/mL; 2 mL gives 50 mg/mL, 5 mL gives 20 mg/mL, 10 mL gives 10 mg/mL.
How many units is 25 mg of NAD+?
At 20 mg/mL (100 mg in 5 mL), 25 mg is 1.25 mL, or 125 units on a U-100 syringe. A different water volume changes this — use the calculator above.
Is there an approved NAD+ dose?
See the published trial doses above. Any human use of a licensed medicine should follow its prescribing information and a prescriber's advice.
What is a NAD+ dosage chart?
A table showing how many syringe units a given amount takes at a given concentration. The reconstitution table on this page is one, calculated for 100 / 500 mg vials.